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產(chǎn)品名稱(chēng)：Invitrogen 10928-034 SuperScript? One-Step RT-PCR System with Platinum? Taq DNA Polymerase
貨 號(hào)：10928-034
規(guī) 格：25 rxns
分 類(lèi)：試劑盒和分析
品 牌：Invitrogen
國(guó)產(chǎn)/進(jìn)口：進(jìn) 口
儲(chǔ)存溫度：-20°C
特惠價(jià)格：致 電
庫(kù)存狀態(tài)：現(xiàn) 貨
銷(xiāo)售商：上?；鄯f生物科技有限公司
慧穎生物專(zhuān)業(yè)供應(yīng)SuperScript? One-Step RT-PCR System with Platinum? Taq DNA Polymerase，常備現(xiàn)貨，*。主營(yíng)Life Invitrogen產(chǎn)品，囊括TRlzol ?Reagent、TRIZOL LS Reagent 、B-27添加劑、凝膠填料、常規(guī)化學(xué)試劑、培養(yǎng)基及原料試劑、各類(lèi)胎牛血清、馬血清等，如有需要，詳詢。
SuperScript? One-Step RT-PCR System with Platinum? Taq DNA Polymerase描述：
The SuperScript? One-Step RT-PCR System with Platinum? Taq DNA Polymerase is designed for the convenient, sensitive, and reproducible detection and analysis of RNA molecules by RT-PCR. Components for both cDNA synthesis and PCR are combined in a single tube, using gene-specific primers and target RNAs from either total RNA or mRNA. Reverse transcription automatically follows PCR cycling without additional steps.
Using the SuperScript? One-Step System
The system consists of two major components: RT/ Platinum? Taq Mix and 2X Reaction Mix. The RT/ Platinum? Taq Mix contains a mixture of SuperScript? II Reverse Transcriptase and Platinum? Taq DNA Polymerase for optimal cDNA synthesis and PCR amplification. SuperScript? II RT is a modified version of Moloney Murine Leukemia Virus (M-MLV) RT, engineered to reduce RNase H activity and increase thermal stability. Platinum? Taq DNA polymerase is Taq DNA polymerase complexed with a proprietary antibody that inhibits polymerase activity at ambient temperatures. The antibody is denatured and polymerase activity is restored during the denaturation step in PCR cycling at 94°C. This provides an automatic "hot start" in PCR, increasing sensitivity, specificity, and yield. The 2X Reaction Mix consists of a proprietary buffer system optimized for reverse transcription and PCR amplification, Mg2+ optimized for universal use, deoxyribonucleotide triphosphates, and stabilizers. This convenient 2X format allows further addition of template and primer at any desired concentration
SuperScript? One-Step RT-PCR System with Platinum? Taq DNA Polymerase同類(lèi)產(chǎn)品主要有:
| 貨號(hào) | 名稱(chēng) | 規(guī)格 |
| 18064-014 | SuperScript? II Reverse Transcriptase | 10,000U |
| 18080-044 | SuperScript? III Reverse Transcriptase | 10,000U |
| 18418-012 | Oligo(dT)12-18 Primer | 25ug |
| 18418-020 | Oligo(dT)20 Primer | 50ul(50uM) |
| 10777-019 | RNaseOUTTM Ribonuclease Inhibitor | 5,000U |
| 18080-051 | SuperScript? III First-Strand | 50 rxn |
| Synthesis System for RT-PCR | ||
| 11668-027 | Lipofectamine TM 2000 Reagent | 0.75ml |
| 11668-019 | 1.5ml | |
| 13778-150 | Lipofectamine RNAiMax | 1.5ml |
| 13778-075 | Lipofectamine RNAiMax | 0.75ml |
| 15596-026 | TRlzol ?Reagent | 100ml |
| 15596-018 | TRlzol ?Reagent | 200ml |
| 10296028 | TRIZOL LS Reagent 200 ml（長(zhǎng)方形） | 200ml |
| 10296010 | TRIZOL LS Reagent 100ml（長(zhǎng)方形） | 100ml |
| 11789020 | BP CLONASE II ENZYME MIX | 20 REACTIONS |
| 11791020 | LR CLONASE II ENZYME MIX | 20 REACTIONS |
| 12328040 | AMV | 100 REACTIONS |
| (CLON AMV FIRST STR SYNTH KIT) | ||
| 28025-013 | M-MLV Reverse Transcriptase | 40,000U |
| 17504-044 | B-27（黃顏色） | 10ml |
| 12587-010 | B-27（黃顏色）不含維生素A | 10ml |
| 15090-046 | 2.5% Trypsin (10X), no Phenol Red | 100ml |
| 15050-065 | 0.25% Trypsin(1X),Phenol Red | 100ml |
| 15050-057 | 500ml | |
| 15140-122 | Pen Strep | 100ml |
| 15140-163 | 20X100ml | |
| 25200-056 | 0.25% Trypsin-EDTA(1X,Phenol Red) | 100ml |
| 25200-114 | 20X100ml | |
| 25300-054 | 0.05% Trypsin(1X),Phenol Red: | 100ml |
| 25300-120 | 20X100ml | |
| 15400-054 | 0.5% Trypsin(10X),: | 100ml |
| 25030-081 | L-Glutamine 200mM | 100ml |
| 25030-164 | 20X100ml | |
| 12328019 | CLONED AMV RT, 750U | TRANSCRIPTASE |
| 12328032 | CLONED AMV FIRST STRA SYN KIT | 25 REACTIONS |
| 10966-018 | Platinum? Taq DNA聚合酶 | 100 rxns |
| 10966-026 | 250 rxns | |
| 10966-034 | 500 rxns | |
| K210007 | PURELINK PLASMID MAXI 25 | 25 REACTIONS |
| 15224041 | T4 DNA LIGASE HI CONC. | 250 U |
| C737303 | ONE SHOT STBL3 COMP E COLI | ONE SHOT STBL3 20 RXN |
| 12033015 | ELECTROMAX DH10B T1 CELLS | 0.5 ML (5 X 0.1 ML) |
| A10460 | ONE SHOT CCDB SURVIVAL 2-T1R | 10 REACTIONS |
| LC5925 | SEEBLUE PLUS2 | 500UL |
| 11708039 | PLATINUM PFX DNA POLYMERASE | 500 REACTIONS #95514- #52806 |
| 12344024 | ACCUPRIME PFX DNA POLYMERASE | 200 REACTIONS |
| 12346086 | ACCUPRIME TAQ HIFI | 200 RXNS |
| 11495017 | PCRX ENHANCER SYSTEM | 250 RXN 52395 |
| 450008 | TOPO XL PCR CLONING KIT | 10 RXN |
| MECOV50 | METHYLCODE BISULFITE CONV KIT | 50 REACTIONS |
| 12935300 | STEALTH RNAI NEG CTL MED GC | 250 μl |
| 2013 | FLUORESCENT OLIGOMER, 20UM | 250 UNITS |
| K159303 | KIT,FAST TRACK | 18 RXN |
| 11917020 | SS DBL STRND CDNA SYN KIT | 50 RXNS |
| LC5677 | MARK12 W RANGE MW STD,1ML | 1 ML |
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